By Dr. Corey Orava

Platelet Rich Plasma

To Freeze, or Not to Freeze

In the spirit of saving their clients’ money, veterinarians frequently inquire “can I freeze PRP?” Unfortunately, there is no simple yes-or-no answer. Arguments can be made supporting both sides, but ultimately the decision must be made by the veterinarian. First, we will examine the arguments supporting the freezing of PRP (platelet rich plasma).

In the Begining

When PRP first became available on the veterinary market the retail price for most kits was in the $250 range, with some products running in excess of $400 per unit. Veterinarians recognize that many clients are price conscious when it comes to veterinary care. Not surprisingly, some veterinarians took to freezing excess PRP in order to save their clients some money. When surveyed to why they froze PRP, the list of answers was rather short, and conspicuously never was it mentioned that the goal was to improve outcomes.

Platelet Activation vs Freezing Platelets

Before we discuss the potential downsides to freezing PRP it is important to address the difference between platelet activation versus freezing platelets.

When evaluating the levels of various growth factors (GFs) in PRP, researchers frequently freeze the samples in order to release the GFs from the platelets. This has the desired effect, but it is not the same mechanism as when platelets undergo activation.
When a blood vessel is damaged, platelets recognize various molecules such as collagen, and become activated.

 

During the activation process, platelets change in shape from roughly spherical to star-like, and actively move their α-granules (organelles full of growth factors) until their membranes merge with the plasma membrane (or indirectly via the open canalicular system) and their contents are secreted.

Activation Process

The majority of the other cytoplasmic contents remain within the platelets. In contrast, when a cell is frozen, ice crystals form within the cytoplasm which tear apart the cell releasing, not only the contents of the α-granules, but everything within the cytoplasm. Thus, the products of activation and freezing are not quite equivalent.

A recent publication on effects of freezing equine PRP confirmed the
above. First, a single freeze cycle was enough to release significant quantities of a catabolic enzyme, MMP-9. This paper also highlighted the problem with most ‘frost-free’ freezers. These units prevent frost build up by undergoing periods where the temperature rises from approximately -20°C to -3°C.

Work by these researchers and others have shown that the following growth factors can decrease with storage: TGF-B, PDGF and IGF-1.

Freezing PRP

Additional Considerations

Another obvious risk of storing PRP is cross-contamination. Any- time humans are involved, errors can occur. Many inks and stickers do not work well under freezer conditions so labels can go missing or get smudged.

Often overlooked, but rather important, are the regulatory considerations around this issue. PRP kits are regulated as medical devices by the FDA. Removing any part of the body, storing it, then returning to same patient on a subsequent visit is equivalent to creating a drug and thus would need prior FDA approval.

Lastly, and possibly most importantly, is efficacy. While there are no in vivo data for our veterinary species, researchers have reviewed studies where Clearly PRP was used in humans with OA there are a variety of issues (osteoarthritis).

Their conclusion: that should be taken into account the best results are associated with
if a veterinarian is considering fresh rather than frozen PRP.

*REFERENCES: Kaux et al│Platelets│2019 (Mar) 27: 1-5
Ljungkvist et al│Haemophilia│2017 May;23(3) McClain and McCarrel│BMC Veterinary Research│2019 (15):292 Milants et al│BioMed Research International│2017, ID 7538604 Roffi et al│Journal of Biomedicine & Biotechnology│2014 (7):692913 Sonker & Dubey│Transfusion Medicine Hemotherapy│2015 May;42(3):174-80